Freezing and drying of biological tissues for electron microscopy.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

how to prepare biological samples and live tissues for scanning electron microscopy (sem)

in this article we review the application and procedures involved in scanning electron microscope (sem) to observe biological and live tissues through using sem at high resolution. we discuss practical methods for optimizing tissue preservation to achieve the two principal goals of biological specimen preparation: (a) preserving biological structures as close to their living configuration as po...

متن کامل

Tandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy.

Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellu...

متن کامل

Scanning Electron Microscopy (SEM) analysis and biological control of Ixodes ricinus using entomopathogenic fungi

In the present study, pathogenicity of four native strains of Entomopathogenic fungus; Metarhizium anisopliae, was studied against adult stage of Ixodes ricinus. For this purpose a total number of 180 adult ticks were examined in triplicate. Thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. Samples were incubated in separate Pet...

متن کامل

Freeze-drying of Biological Specimens for Electron Microscopy Using Thermoelectric Cooling

Freeze-drying for electron microscopy is one of the most successful methods for avoiding specimen distortion caused by drying of liquid solutions in air. It has been calculated that the surface forces of receding droplets subject small specimens to pressures of the order of tons per square inch (1). In freeze-drying, an aqueous solution of the specimen is rapidly frozen and the ice allowed to s...

متن کامل

Electron Microscopy of Cells and Tissues

I read this book the same weekend that the Packers took on the Rams, and the experience of the latter event, obviously, colored my judgment. Although I abhor anything that smacks of being a handbook (like, "How to Earn a Merit Badge in Neurosurgery") because too many volumes in biomedical science already evince a boyscout-like approach, I must confess that parts of this volume are fast, scholar...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Histochemistry & Cytochemistry

سال: 1981

ISSN: 0022-1554,1551-5044

DOI: 10.1177/29.9.7026665